Analysis Note

ControlRat oligodendrocyte precursor cells

Application

Research CategoryNeuroscience

Research Sub CategoryDevelopmental Neuroscience

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Oligodendrocyte lineage transcription factor 2 (Olig2) is a protein belonging to group A of the OLIG family of basic helix-loop-helix transcription factors (bHLH). bHLH proteins are divided into 6 groups (A-F), and characterized by their biochemical properties and structure. Olig2 is classified as group A due to its ability to form heterodimers with other bHLH proteins and binding to the E box. Olig2 has a crucial role during development in specifying the final location of motor neurons and oligodendrocytes in the spinal cord, along with the development within the hindbrain of somatic motor neurons. Olig2 expression is observed within oligodendrocytes and in developing astrocytes. Recent studies have shown strong expression of Olig2 in human oligodendrogliomas, primary tumors within the central nervous system, and moderate expression in astrocytomas. These observations have led to the proposal of Olig2 having potential as an oligodendroglioma diagnostic marker.

Immunogen

Recombinant protein corresponding to human Olig2.

Legal Information

ALEXA FLUOR is a registered trademark of Life Technologies

Physical form

Purified mouse monoclonal IgG2a conjugated to Alexa Fluor^® 488 in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Protein G Purified

Quality

Evaluated by Immunocytochemistry in rat oligodendrocyte precursor cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Olig2 in rat oligodendrocyte precursor cells.

Storage and Stability

Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Target description

The unconjugated parent antibody (Cat. No. MABN50) has an observed molecular weight at ~37 kDa